Last year, researchers announced one of the most promising methods yet for creating ethically neutral stem cells: reprogramming adult human cells to act like embryonic stem cells. This involved using four transcription factor proteins to turn specific genes on and off. But the resulting cells, called induced pluripotent stem (iPS) cells for their ability to develop into just about any tissue, have one huge flaw. They’re made with a virus that embeds itself into the cells’ DNA and, over time, can induce cancer. Now, scientists at Harvard University have found a way to effect the same reprogramming without using a harmful virus–a method that paves the way for tissue transplants made from a patient’s own cells.
The first generation of iPS cells was created using a retrovirus to insert the four transcription factors into skin cells. Because a retrovirus, by definition, inserts itself permanently into its host’s DNA, this ensured that the transcription factors were transferred, but it also led to the propagation of the virus itself. Furthermore, since the virus confers self-renewal capabilities to its new host cell, many believed that the retrovirus might be required for iPS cells to reproduce.
New research by Konrad Hochedlinger and his colleagues at Harvard University, the Harvard Stem Cell Institute, and the MGH Center for Regenerative Medicine shows that a different type of virus–an adenovirus–can make the transfer in mouse cells without permanently integrating itself. The resulting iPS cells can divide indefinitely but show no trace of the virus–just a temporary infection that disappears within a short time. “That means that the four transcription factors themselves are sufficient to induce pluripotency in adult cells,” Hochedlinger says.